(주)신영코퍼레이션

In the center of molecular biology is one species of molecules: DNA.: DNA molecules are amplified and introduced into organisms by transformation or transfection, separated, stained, examined under the microscope, manipulated, sequenced and so on.
For all these techniques the initial step is to isolate DNA from the origin of interest.
This page provides you an overview of the different methods for nucleic acid isolation and offers a number of must-have reagents to obtain pure DNA and/or RNA from various sources.

Introduction
What does it mean technically when we talk about DNA extraction, isolation or purification - terms often used synonymously for getting preferably pure DNA from a sample? What are the mechanisms behind? How is it possible to separate distinct species of DNA?
On this page we focus on the isolation of the two DNA species that are mainly isolated in molecular biology labs - genomic DNA and bacterial plasmids. We give an overview about established DNA isolation techniques, their chemical background and we discuss their respective advantages and limitations.

Regarding the basic procedure, DNA extraction is simple and can be done using domestic products. Basically, all you require is a rich source of DNA, salt, water, dishwashing detergent, a coffee filter, high-proof alcohol and a stick to spool the precipitated DNA salt out of solution. For higher demands (regarding quantity and quality), of course, the method requires further refinement.

Catalog	Description	Packaging	Specification
A7248	Dimethyl Sulfoxide, sterile filtered (ampules) 67-68-5	5 x 10 ml	CAS No. 67-68-5 / Liquid / M.W 78.13 g/mol
A5456	Tetramethylammonium Chloride solution (6 M) for molecular biology	500 ml	DNases/RNases/Proteases: not detectable Composition: TMACl: 657 g/L
A5187	DNA Phage Lambda	1 mg	Concentration: 0.2 - 0.5 mg/mL
A1152	Ethidium Bromide solution 1 % BioChemica 1239-45-8	10 ml / 25 ml / 100 ml	CAS No. 1239-45-8 / Liquid / M.W 394.33 g/mol
A8368	DNA Ladder 50 bp	50 μg	• Number of bands: 10 • Fragment sizes in bp: 700; 500; 400; 350; 300 (x2); 250; 200; 150; 100; 50 Concentration: 0.1 mg/mL
A5324	Magnesium Chloride solution (100 mM) for molecular biology	1 ml	DNases/RNases/Proteases: not detectable Concentration: 100 mM
A3276	Phenol liquid non water-saturated, non stabilized BioChemica 108-95-2	100 ml / 1 L	CAS No. 108-95-2 / Liquid
A1126	Cesium Chloride 99.9 % BioChemica 7647-17-8	100 g / 1 kg	CAS No. 7647-17-8 / Solid / M.W 168.36 g/mol
A8510	Water PCR tested, DNA free, for molecular biology, 7732-18-5	10 x 1,7 ml	CAS No. 7732-18-5 / Liquid / M.W 18.01 g/mol
A5216	DNA Ladder 100 bp plus	50 μg / 250 μg	• Fragment sizes in bp: 1500; 1000 (x2); 900; 800; 700; 600; 500 (x2); 400; 300; 200; 100 • Number of bands: 11 Concentration: 0.2 mg/mL

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