(주)신영코퍼레이션

Biochemical processes are markedly impaired by even small changes in the concentrations of free H+ ions.: It is therefore usually required to stabilise the H + concentration in vitro without affecting the system's functioning.
A buffer keeps the pH of a solution constant by taking up protons that are released during reactions, or by releasing protons when they are consumed by reactions.
The observation that partially neutralised solutions of weak acids or bases are resistant to changes in pH when small amounts of strong acids or bases are added led to the concept of the ‘buffer’. In the table, the most common biological buffers are listed according to their optimal buffer range.
The table also gives some hints for potential applications and shows advantages or disadvantages of the listed buffer substances.

Buffer systems described in the literature are usually used for experiments to enable direct comparison of results.
Again and again, it is shown that the conditions in experiments - even in standard systems - could be optimised.

In our Info Point "Biological Buffers" we provide a selection of important information from the literature that might help you in solving your everyday problems as well as give recommendations for the development and optimization of your test systems: Which requirements do biological buffers have to fulfil? Which criteria need to be taken into account? How is the pH value adjusted effectively?

Catalog	Description	Packaging	Specification
A4335	Guanidine Thiocyanate BioChemica 593-84-0	1 kg	CAS No. 593-84-0 / Solid / M.W 118.16 g/mol
A4263	Tris buffer pH 7.5 (1 M) for molecular biology	500 ml	DNases/RNases/Proteases: not detectable pH (20°C; adjusted with HCl): 7.5 ± 0.2 Composition: Tris: 121.14 g/L (1 M)
A3981	Tris buffer pH 7.4 (1 M) for molecular biology	1 L	DNases/RNases/Proteases: not detectable pH (20°C; adjusted with HCl): 7.4 ± 0.1 Composition: Tris: 121.14 g/L (1 M)
A3981	Tris buffer pH 7.4 (1 M) for molecular biology	1 L	DNases/RNases/Proteases: not detectable pH (20°C; adjusted with HCl): 7.4 ± 0.1 Composition: Tris: 121.14 g/L (1 M)
A3954	Tricine for molecular biology 5704-04-1	500 g	CAS No. 5704-04-1 / Solid / M.W 179.17 g/mol
A3945	TBE buffer (10X) for molecular biology	1 L	DNases/RNases/Proteases: not detectable pH (20°C; H2O): 8.3 ± 0.2 Composition: Boric Acid: 55.03 g/L (0.89 M) EDTA-Na2 · 2H2O: 7.44 g/L (0.02 M) Tris: 107.81 g/L (0.89 M)
A3906	di-Sodium Hydrogen Phosphate 12-hydrate BioChemica 10039-32-4	1 kg	CAS No. 10039-32-4 / Solid / M.W 358.14 g/mol
A3905	di-Sodium hydrogen phosphate 2-hydrate BioChemica 10028-24-7	1 kg	CAS No. 10028-24-7 / Solid / M.W 177.99 g/mol
A3901	tri-Sodium Citrate 2-hydrate BioChemica 6132-04-3	1 kg / 25 kg	CAS No. 6132-04-3 / Solid / M.W 294.10 g/mol
A3724	HEPES for molecular biology 7365-45-9	250 g / 500 g / 1 kg	CAS No. 7365-45-9 / Solid / M.W 238.31 g/mol

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