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BuffersDetergentsChemicals for Bioresearch
Biochemical processes are markedly impaired by even small changes in the concentrations of free H+ ions.
It is therefore usually required to stabilise the H + concentration in vitro without affecting the system's functioning.
A buffer keeps the pH of a solution constant by taking up protons that are released during reactions, or by releasing protons when they are consumed by reactions.
The observation that partially neutralised solutions of weak acids or bases are resistant to changes in pH when small amounts of strong acids or bases are added led to the concept of the ‘buffer’. In the table, the most common biological buffers are listed according to their optimal buffer range.
The table also gives some hints for potential applications and shows advantages or disadvantages of the listed buffer substances.

Buffer systems described in the literature are usually used for experiments to enable direct comparison of results.
Again and again, it is shown that the conditions in experiments - even in standard systems - could be optimised.

In our Info Point "Biological Buffers" we provide a selection of important information from the literature that might help you in solving your everyday problems as well as give recommendations for the development and optimization of your test systems: Which requirements do biological buffers have to fulfil? Which criteria need to be taken into account? How is the pH value adjusted effectively?
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전체선택
Thumbnail Catalog Description Packaging Specification
A1085 Tricine BioChemica 5704-04-1 250 g / 500 g / 1 kg / 5 kg CAS No. 5704-04-1 / Solid / M.W 179.17 g/mol
A0972 TBE buffer (10X) 1 L / 5 L / 10 L / pH (20°C; H2O): 8.3 ± 0.2 Composition: Boric Acid: 55.03 g/L (0.89 M) EDTA-Na2 · 2H2O: 7.44 g/L (0.02 M) Tris: 107.81 g/L (0.89 M)
A0697 Trifluoroacetic acid BioChemica 76-05-1 100 ml CAS No. 76-05-1 / Liquid / M.W 114.02 g/mol
A9031 TE buffer (1X) pH 7.4 for molecular biology 5 L DNases/RNases/Proteases: not detectable pH (20°C; adjusted with HCl): 7.4 ± 0.1 Composition: EDTA · Na2 · 2H2O: 0.372 g/L (1 mM) Tris: 1.211 g/L (10 mM)
A6916 HEPES buffer pH 7.5 (1 M) sterile 125 ml / 250 ml pH (20°C; adjusted with NaOH): 7.5 ± 0.2 Composition: HEPES: 238.31 g/L (1 M)
A4228 TBE buffer (5X) for molecular biology 5 L DNases/RNases/Proteases: not detectable pH (20°C; H2O): 8.3 ± 0.2 Composition: Boric Acid: 27.52 g/L (0.445 M) EDTA-Na2 · 2H2O: 3.72 g/L (0.01 M) Tris: 53.91 g/L (0.445 M)
A3945 TBE buffer (10X) for molecular biology 1 L DNases/RNases/Proteases: not detectable pH (20°C; H2O): 8.3 ± 0.2 Composition: Boric Acid: 55.03 g/L (0.89 M) EDTA-Na2 · 2H2O: 7.44 g/L (0.02 M) Tris: 107.81 g/L (0.89 M)
A3724 HEPES for molecular biology 7365-45-9 250 g / 500 g / 1 kg CAS No. 7365-45-9 / Solid / M.W 238.31 g/mol
A1106 Guanidine Hydrochloride for molecular biology 50-01-1 1 kg CAS No. 50-01-1 / Solid / M.W 95.53 g/mol
A1086 Tris ultrapure 77-86-1 500 g / 1 kg / 5 kg / 10 kg / 25 kg CAS No. 77-86-1 / Solid / M.W 121.14 g/mol

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