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Biochemical processes are markedly impaired by even small changes in the concentrations of free H+ ions.
It is therefore usually required to stabilise the H + concentration in vitro without affecting the system's functioning.
A buffer keeps the pH of a solution constant by taking up protons that are released during reactions, or by releasing protons when they are consumed by reactions.
The observation that partially neutralised solutions of weak acids or bases are resistant to changes in pH when small amounts of strong acids or bases are added led to the concept of the ¡®buffer¡¯. In the table, the most common biological buffers are listed according to their optimal buffer range.
The table also gives some hints for potential applications and shows advantages or disadvantages of the listed buffer substances.

Buffer systems described in the literature are usually used for experiments to enable direct comparison of results.
Again and again, it is shown that the conditions in experiments - even in standard systems - could be optimised.

In our Info Point "Biological Buffers" we provide a selection of important information from the literature that might help you in solving your everyday problems as well as give recommendations for the development and optimization of your test systems: Which requirements do biological buffers have to fulfil? Which criteria need to be taken into account? How is the pH value adjusted effectively?
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Thumbnail Catalog Description Packaging Specification
A2140 Cacodylic Acid Sodium Salt 3-hydrate BioChemica 6131-99-3 100 g / 250 g CAS No. 6131-99-3 / Solid / M.W 214.03 g/mol
A1087 Tris Hydrochloride for buffer solutions 1185-53-1 250 g / 500 g / 1 kg / 5 kg CAS No. 1185-53-1 / Solid / M.W 157.60 g/mol
A0703 Guanidine Thiocyanate solution (6 M in 0.1 M Tris; pH 7.5) for molecular biology 1 L DNases/RNases/Proteases: not detectable pH (25¡ÆC): 7.3 - 7.7 Composition: Tris: 12.11 g/L GuaSCN: 708.96 g/L
A0703 Guanidine Thiocyanate solution (6 M in 0.1 M Tris; pH 7.5) for molecular biology 1 L DNases/RNases/Proteases: not detectable pH (25¡ÆC): 7.3 - 7.7 Composition: Tris: 12.11 g/L GuaSCN: 708.96 g/L
A3240 Guanidine Hydrochloride ultrapure 50-01-1 500 g / 1 kg / 5 kg CAS No. 50-01-1 / Solid / M.W 95.53 g/mol
A1378 Imidazole for molecular biology 288-32-4 50 g / 250 g CAS No. 288-32-4 / Solid / M.W 68.08 g/mol
A1135 Bis-Tris-Propane for buffer solutions 64431-96-5 100 g / 500 g CAS No. 64431-96-5 / Solid / M.W 282.34 g/mol
A1025 Bis-Tris for buffer solutions 6976-37-0 100 g / 250 g / 500 g / 1 kg CAS No. 6976-37-0 / Solid / M.W 209.24 g/mol
A0590 Trichloroacetic Acid solution 20 % BioChemica 500 ml / 1 L Assay (titr.): 19.95 - 20.05 % pH (20¡ÆC): <1.0
A1107 Guanidine Thiocyanate for molecular biology 593-84-0 100 g / 250 g / 500 g / 1 kg / 25 kg CAS No. 593-84-0 / Solid / M.W 118.16 g/mol

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